13FR
Cholesterol complex of the START domain of Caenorhabditis elegans StAR-related lipid transfer protein 3 (STARD3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-30 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.976230 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.512, 77.667, 85.294 |
| Unit cell angles | 90.00, 116.17, 90.00 |
Refinement procedure
| Resolution | 29.370 - 2.090 |
| R-factor | 0.2119 |
| Rwork | 0.210 |
| R-free | 0.24310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.642 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.370 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.107 | 0.636 |
| Rmeas | 0.125 | 0.768 |
| Rpim | 0.065 | 0.422 |
| Number of reflections | 57282 | 4398 |
| <I/σ(I)> | 9.7 | 4.1 |
| Completeness [%] | 97.6 | 97.6 |
| Redundancy | 3.4 | 3.2 |
| CC(1/2) | 0.986 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 50 mM sodium cacodylate pH 6.5, 100 mM magnesium acetate, 200 mM potassium chloride, 14% w/v PEG 8000 |
