12YI
The GNMT N-Terminal Domain Orchestrates Folate-Dependent Regulation of Cellular Methylation Dynamics
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-10-22 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.067, 144.147, 98.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 1.650 |
| R-factor | 0.2036 |
| Rwork | 0.170 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.878 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.130 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.044 | 0.219 |
| Rmeas | 0.052 | 0.263 |
| Rpim | 0.027 | 0.143 |
| Total number of observations | 499083 | 22380 |
| Number of reflections | 145249 | 6827 |
| <I/σ(I)> | 13.5 | 3.6 |
| Completeness [%] | 97.0 | |
| Redundancy | 3.4 | 3.3 |
| CC(1/2) | 0.998 | 0.945 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M ammonium formate and 20% (w/v) PEG 3350 |
