12UO

BCL11B ZF2-3 in Complex with a DNA Sequence Observed in the Human Globin Locus Containing Motif TGGCCA (Oligo 4-3)-C2 Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-09-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919901
Spacegroup name	C 1 2 1
Unit cell lengths	73.818, 241.077, 45.698
Unit cell angles	90.00, 128.24, 90.00

Refinement procedure

Resolution	19.860 - 3.050
R-factor	0.2406
Rwork	0.239
R-free	0.26930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.639
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX (2.0_5885)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.860	3.260
High resolution limit [Å]	3.050	3.050
Rmerge	0.160
Rpim	0.065	0.626
Number of reflections	23548	4242
<I/σ(I)>	7.3	1.1
Completeness [%]	98.9	98.7
Redundancy	7	7.3
CC(1/2)	0.999	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	0.2 M DL-Malic acid pH 7.0, 20% w/v Polyethylene glycol 3,350