12UN
BCL11B ZF2-3 in Complex with a DNA Sequence Observed in the Human Globin Locus Containing Motif TGGCCA (Oligo 4-3)-P212121 Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-09-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.919901 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.444, 49.026, 234.564 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.890 - 3.030 |
| R-factor | 0.2253 |
| Rwork | 0.224 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.699 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (2.0_5885) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | 3.080 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Rmerge | 0.181 | |
| Rpim | 0.052 | |
| Number of reflections | 21664 | 1026 |
| <I/σ(I)> | 9.9 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 13.6 |
| CC(1/2) | 0.999 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 45% v/v Polypropylene glycol P 400, 0.1 M BIS-TRIS pH 6.5 |
