12UM

BCL11B ZF2-3 in Complex with a DNA Sequence Observed in the Human Globin Locus Containing Motif TGTCCA (Oligo 3-5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-08-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919716
Spacegroup name	P 65
Unit cell lengths	39.575, 39.575, 201.688
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.790 - 1.930
R-factor	0.19
Rwork	0.189
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.899
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (2.0_5885)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.790	1.970
High resolution limit [Å]	1.930	1.930
Rmerge	0.126
Rpim	0.029
Number of reflections	26498	1399
<I/σ(I)>	14.1	0.9
Completeness [%]	100.0	100
Redundancy	20.2	20.4
CC(1/2)	0.997	0.364

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	292	26 % w/v PEG 2000 MME, 0.1 M Bis-Tris 5.8