12UL

BCL11B ZF2-3 in Complex with a DNA Sequence Observed in the Human Globin Locus Containing Motif TGTCCA (Oligo 3-4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919716
Spacegroup name	P 41 21 2
Unit cell lengths	50.486, 50.486, 169.298
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.890 - 2.810
R-factor	0.2272
Rwork	0.226
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.535
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (2.0_5885)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.890	2.860
High resolution limit [Å]	2.810	2.810
Rpim	0.075	1.680
Number of reflections	11712	896
<I/σ(I)>	10.5	0.9
Completeness [%]	100.0
Redundancy	25.2
CC(1/2)	0.999	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	292	28% PEG 4000, 0.1M TRIS-HCl pH 8.0