12RU
In situ cryo-EM structure of axonal microtubules from ghost neurons (expanded lattice)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.66 |
| Number of Particle | 158108 |
| Software | cryoSPARC,PHENIX,FREALIGN,SerialEM,UCSF ChimeraX,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
