12QA
Crystal structure of CM10 Fab in complex with an orthomarburgvirus GP2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.222, 41.687, 80.643 |
| Unit cell angles | 90.00, 102.17, 90.00 |
Refinement procedure
| Resolution | 36.850 - 1.600 |
| R-factor | 0.1766 |
| Rwork | 0.175 |
| R-free | 0.20720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.391 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.658 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.122 | 0.618 |
| Number of reflections | 50772 | 3822 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 87.5 | |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 3% DMSO, 30%(v/v) MPD, 15%(w/v) PEG 8000, 0.1M Calcium Chloride, 0.1M Sodium Acetate/Acetic Acid pH 4.5 |
