12OA

Crystal structure of a GH26 enzyme (EiGH26c)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRIUS BEAMLINE MANACA
Synchrotron site	LNLS SIRIUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-09-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.977200
Spacegroup name	P 21 21 2
Unit cell lengths	70.468, 210.750, 63.733
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.120 - 1.770
R-factor	0.1822
Rwork	0.181
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.011
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.120	46.120	1.870
High resolution limit [Å]	1.770	5.270	1.770
Rmerge	0.139	0.028	3.085
Rmeas	0.182	0.036	4.119
Number of reflections	179637	3147	13809
<I/σ(I)>	3.84
Completeness [%]	47.2
Redundancy	6.777
CC(1/2)	0.993	0.998	0.085

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1M (NH4)2SO4