12NY

Crystal structure of a GH26 enzyme (EiGH26b) in complex with mannose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRIUS BEAMLINE MANACA
Synchrotron site	LNLS SIRIUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.977200
Spacegroup name	P 43 2 2
Unit cell lengths	87.007, 87.007, 85.414
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.500 - 1.710
R-factor	0.1763
Rwork	0.175
R-free	0.20190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.307
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.500	43.500	1.820
High resolution limit [Å]	1.710	5.110	1.710
Rmerge	0.089	0.032	0.800
Rmeas	0.113	0.038	1.049
Number of reflections	35159	766	4003
<I/σ(I)>	6.94
Completeness [%]	68.7
Redundancy	13.08
CC(1/2)	0.995	0.998	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	0.1 M Sodium Acetate 4.6 pH, 2 M (NH4)2SO4