12NP
Crystal structure of the CD7 ectodomain bound to K12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2026-02-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.681, 121.950, 88.871 |
| Unit cell angles | 90.00, 102.99, 90.00 |
Refinement procedure
| Resolution | 44.990 - 2.400 |
| R-factor | 0.2431 |
| Rwork | 0.241 |
| R-free | 0.27810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.129 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER (2.0_5885) |
| Refinement software | PHENIX (2.0_5885) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.990 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.072 | 0.442 |
| Rmeas | 0.093 | 0.575 |
| Rpim | 0.059 | 0.362 |
| Number of reflections | 40244 | 4229 |
| <I/σ(I)> | 10.5 | 2.7 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.4 | 4.49 |
| CC(1/2) | 0.995 | 0.835 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.2M MgCl2, 0.1M Tris (pH=7.0), 6% w/v PEG8000 |
