12HG
Crystal Structure of the N-terminal Domain of the Nsp8 from SARS-CoV2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-14 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.879, 56.272, 81.043 |
| Unit cell angles | 90.00, 92.73, 90.00 |
Refinement procedure
| Resolution | 40.590 - 2.350 |
| R-factor | 0.2244 |
| Rwork | 0.223 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.460 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.176 | 0.687 |
| Rmeas | 0.192 | |
| Rpim | 0.075 | 0.303 |
| Number of reflections | 30760 | 1501 |
| <I/σ(I)> | 14.98 | 2 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 6.6 | 5.9 |
| CC(1/2) | 0.955 | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M citric acid, pH 4.0, 2.5 M NaCl |
