126L
THE ENERGETIC COST AND THE STRUCTURAL CONSEQUENCES OF BURYING A HYDROXYL GROUP WITHIN THE CORE OF A PROTEIN DETERMINED FROM ALA TO SER AND VAL TO THR SUBSTITUTIONS IN T4 LYSOZYME
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.000, 61.000, 96.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 1.800 |
| R-factor | 0.155 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.900 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.033 * |
| Number of reflections | 17805 * |
| Completeness [%] | 87.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.9 * | 4 * | Matthew, B.W., (1973) J. Mol. Biol., 78, 575. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | potassium phosphate | 1.1 (M) | |
| 2 | 1 | 1 | sodium phosphate | 1.1 (M) | |
| 3 | 1 | 1 | 0.15 (M) | ||
| 4 | 1 | 1 | mercaptoethanol | 1.4 (mM) | |
| 5 | 1 | 1 | protain | 3.5 (mg/ml) |
