11YY
E.Coli DNA Topoisomerase 3 in complex with an 8mer ssDNA oligo ACTGACTT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2026-03-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.912, 132.372, 78.777 |
| Unit cell angles | 90.00, 134.24, 90.00 |
Refinement procedure
| Resolution | 56.440 - 2.200 |
| R-factor | 0.2176 |
| Rwork | 0.217 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.563 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.440 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.094 | 1.052 |
| Rmeas | 0.103 | 1.160 |
| Rpim | 0.043 | 0.478 |
| Number of reflections | 40486 | 3006 |
| <I/σ(I)> | 8.8 | 1.3 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 5.7 | 5.7 |
| CC(1/2) | 0.988 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 289 | 0.2M Ammonium Citrate Dibasic, 20% (w/v) PEG3350 |
