11NY

Crystal Structure of viral OTU domain protease from Tacheng Tick Virus 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 21 21 21
Unit cell lengths	47.767, 58.070, 59.256
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.630 - 1.370
R-factor	0.1634
Rwork	0.161
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.237
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (2.0_5936)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.630	1.389
High resolution limit [Å]	1.366	1.366
Number of reflections	35646	3392
<I/σ(I)>	1.778
Completeness [%]	97.7
Redundancy	5.9
CC(1/2)	0.997	0.284

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	0.1 M Lithium sullfate, 21 % PEG 3350, 0.1M Bis-tris pH 6.5