11MZ

Influenza A virus Hemagglutinin (A/California/04/2009 H1N1), E47K HA2 stabilizing mutation (C3 symmetry) determined using the SPT Labtech chameleon in the presence of 0.25x SurfACT

EM specimen

Vitrification	Yes
Instrument	SPT LABTECH CHAMELEON

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	TFS Selectris X
Detector	TFS FALCON 4i (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.64
Software	cryoSPARC,PHENIX,EPU
Symmetry	C3
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION