11LB
E.Coli DNA Topoisomerase 3 in complex with an 8mer ssDNA oligo GCAACTGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-11-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.685, 120.884, 85.609 |
| Unit cell angles | 90.00, 99.34, 90.00 |
Refinement procedure
| Resolution | 60.440 - 1.850 |
| R-factor | 0.1795 |
| Rwork | 0.178 |
| R-free | 0.21180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.141 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.500 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.097 | 0.917 |
| Rmeas | 0.110 | 1.040 |
| Rpim | 0.051 | 0.481 |
| Number of reflections | 64910 | 4763 |
| <I/σ(I)> | 8.2 | 1.5 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 4.5 | 4.6 |
| CC(1/2) | 0.996 | 0.454 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M Sodium Citrate, 20%(w/V)PEG 4000, 10% (v/v) 2-Propanol |
