11KR
FabG1 targeting prion oligomers and fibrils
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-27 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 31 |
| Unit cell lengths | 71.754, 71.754, 89.505 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.320 - 2.480 |
| R-factor | 0.1888 |
| Rwork | 0.186 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.978 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0_5936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.320 | 2.620 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 134886 | 5233 |
| <I/σ(I)> | 0.68 | |
| Completeness [%] | 99.2 | 94.82 |
| Redundancy | 17.6 | 7.1 |
| CC(1/2) | 0.972 | 0.009 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.5 | 293.15 | 0.2M Zinc acetate, 0.1M Sodium acetate pH 4.5, 10% PEG 3000 |
