11KB
E.Coli DNA Topoisomerase 3 in complex with an 8mer ssDNA oligo AACTGTTG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-10-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.966, 123.124, 86.255 |
| Unit cell angles | 90.00, 98.67, 90.00 |
Refinement procedure
| Resolution | 42.640 - 1.900 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.925 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.700 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.095 | 0.906 |
| Rmeas | 0.126 | 1.200 |
| Rpim | 0.065 | 0.576 |
| Number of reflections | 63110 | 4699 |
| <I/σ(I)> | 6.2 | 1.3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.985 | 0.618 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M Lithium Acetate, 20%(w/v) PEG3350 |
