11IZ

Crystal structure of HSC-HSC-AMS bound DesD, the desferrioxamine synthetase from the Streptomyces griseoflavus ferrimycin biosynthetic pathway

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	113
Detector technology	PIXEL
Collection date	2025-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97935
Spacegroup name	C 2 2 21
Unit cell lengths	128.643, 236.822, 331.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.450 - 2.470
R-factor	0.1768
Rwork	0.175
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.139
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.450	2.610
High resolution limit [Å]	2.470	2.470
Rmerge	0.267	1.660
Number of reflections	179121	25958
<I/σ(I)>	7.66
Completeness [%]	100.0
Redundancy	14.1
CC(1/2)	0.990	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	287	0.05M Sodium phosphate pH 7.0 0.2M Ammonium sulfate 16% PEG 4000