11GX
E.Coli DNA Topoisomerase 3 in complex with an 8mer ssDNA oligo CGCAACTT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-07-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.385, 132.520, 77.321 |
| Unit cell angles | 90.00, 133.96, 90.00 |
Refinement procedure
| Resolution | 42.620 - 2.220 |
| R-factor | 0.2224 |
| Rwork | 0.221 |
| R-free | 0.26050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.584 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.700 | 2.280 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.058 | 1.037 |
| Rmeas | 0.068 | 1.198 |
| Rpim | 0.034 | 0.594 |
| Number of reflections | 39262 | 2897 |
| <I/σ(I)> | 10.3 | 1 |
| Completeness [%] | 98.9 | 99.2 |
| Redundancy | 3.9 | 3.9 |
| CC(1/2) | 0.999 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M Sodium Citrate, 20%(w/v) PEG4000, 20% (v/v) 2-Propanol |
