11FC

E.Coli DNA Topoisomerase 3 in complex with an 8mer ssDNA oligo CTGAACTT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-02-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9787
Spacegroup name	C 1 2 1
Unit cell lengths	109.794, 133.242, 78.434
Unit cell angles	90.00, 133.98, 90.00

Refinement procedure

Resolution	34.310 - 2.100
R-factor	0.2291
Rwork	0.227
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.503
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.400	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.092	0.767
Rmeas	0.109	0.922
Rpim	0.058	0.507
Number of reflections	47324	3478
<I/σ(I)>	8.6	1.5
Completeness [%]	99.9	99.6
Redundancy	6.7	6.2
CC(1/2)	0.997	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		286	0.2M Ammonium Citrate Dibasic, 20% (w/v) PEG 3350