11BD

human nucleosome assembly protein 1 (human Nap1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	170.610, 170.610, 60.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.150 - 3.200
R-factor	0.2306
Rwork	0.227
R-free	0.26650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.904
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.150	3.420
High resolution limit [Å]	3.200	3.200
Rmerge	0.069	0.202
Number of reflections	13151	1836
<I/σ(I)>	6.5	1
Completeness [%]	96.8
Redundancy	1.8
CC(1/2)	0.940	0.200

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	26% PEG 400, 0.1 M sodium citrate, pH 4.5, 0.1 M magnesium chloride hexahydrate, 0.1 M sodium chloride, 2% 1,4-dioxane