11AO
Crystal structure of mouse DXO in complex with the oligonucleotide pA5 with 5'phosphate in the product and substrate states
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97905 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.996, 88.253, 53.142 |
| Unit cell angles | 90.00, 113.18, 90.00 |
Refinement procedure
| Resolution | 48.850 - 1.590 |
| R-factor | 0.1816 |
| Rwork | 0.181 |
| R-free | 0.18950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.490 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.850 | 1.630 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Number of reflections | 56497 | 9073 |
| <I/σ(I)> | 13.54 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.998 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% (w/v) PEG 3350 and 0.1 M Tris (pH 6.8). |
