11AF
Crystal structure of JADE3 PZP domain in complex with Histone H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-06-03 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 35.887, 98.415, 104.426 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.590 - 2.600 |
| R-factor | 0.23422 |
| Rwork | 0.232 |
| R-free | 0.27762 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.795 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.120 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 1.046 | |
| Rmeas | 0.373 | 1.156 |
| Rpim | 0.152 | 0.484 |
| Total number of observations | 7967 | |
| Number of reflections | 11979 | 1413 |
| <I/σ(I)> | 5.7 | 1.8 |
| Completeness [%] | 99.8 | |
| Redundancy | 5.9 | 5.6 |
| CC(1/2) | 0.957 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M HEPES pH 8.5 and 25% PEG 5000 |
