11PI
Crystal structure of UFC1 variant Y42F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2026-02-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 46.124, 46.124, 142.715 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.650 - 1.430 |
| R-factor | 0.1775 |
| Rwork | 0.176 |
| R-free | 0.19740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.177 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | BUSTER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.900 | 1.530 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rpim | 0.026 | 0.817 |
| Number of reflections | 25276 | 620 |
| <I/σ(I)> | 10.3 | 0.5 |
| Completeness [%] | 86.2 | 43.4 |
| Redundancy | 17.1 | 2.6 |
| CC(1/2) | 0.999 | 0.395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 294 | 0.2 M LiSO4, 1.2 M NaH2PO4, 0.8 M K2HPO4, 0.1 M CAPS pH 10.5 |
