117D
CRYSTAL AND MOLECULAR STRUCTURE OF THE ALTERNATING DODECAMER D(GCGTACGTACGC) IN THE A-DNA FORM: COMPARISON WITH THE ISOMORPHOUS NON-ALTERNATING DODECAMER D(CCGTACGTACGG)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-6 |
| Detector technology | OSCILLATION CAMERA |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 46.200, 46.200, 71.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.550 |
| R-factor | 0.142 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 0.045 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.550 |
| Rmerge | 0.050 * |
| Total number of observations | 2008 * |
| Number of reflections | 1115 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | pH 6.50, VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | SPERMINE | ||
| 3 | 1 | 1 | [CO(NH3)6]3+ | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | PROPANOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA duplex | 1 (mM) | |
| 2 | 1 | drop | spermine | 1 (mM) | |
| 3 | 1 | drop | cobalt hexammine | 3 (mM) | |
| 4 | 1 | reservoir | 1-propanol | 30 (%) |
