111D
CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | DIFFRACTOMETER |
| Detector | STOE-SIEMENS AED2 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.230, 41.160, 65.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.250 |
| R-factor | 0.16 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 2840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.6 | 4 * | pH 6.60, VAPOR DIFFUSION, SITTING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | DNA | 0.5 (mM) | ||
| 2 | 1 | 2 | sodium cacodylate | 50 (mM) | |
| 3 | 1 | 2 | magnesium chloride | 20 (mM) | |
| 4 | 1 | 2 | spermine | 1.0 (mM) | |
| 5 | 1 | 2 | MPD | 18 (%(v/v)) |
