10ZO
Crystal structure of Mengla virus nucleoprotein bound by a cross-reactive anti-Marburg virus nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-08-13 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54178 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 214.284, 214.284, 57.338 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.060 - 1.650 |
| R-factor | 0.1651 |
| Rwork | 0.163 |
| R-free | 0.21430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.794 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0_5936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.390 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rpim | 0.030 | 0.583 |
| Number of reflections | 60116 | 8744 |
| <I/σ(I)> | 15.4 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 10.4 |
| CC(1/2) | 0.999 | 0.492 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M sodium malonate dibasic monohydrate; 0.1 M HEPES pH 7; 30% w/v poly(acrylic acid sodium salt) 2100 |
