10ZN
CryoEM structure of Heterologous nitrogenase complex (2:1 A. vinelandii-FeP:G. diazotrophicus-MoFeP) inhibited by BeFx (C2 symmetry)
EM specimen
| Vitrification | Yes |
| Instrument | SPT LABTECH CHAMELEON |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | TFS Selectris X |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.92 |
| Number of Particle | 281613 |
| Software | cryoSPARC,EPU,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
