10YS

Structure of Cbl-B bound to compound 33

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	57.275, 74.032, 106.800
Unit cell angles	90.00, 93.68, 90.00

Refinement procedure

Resolution	60.800 - 2.300
R-factor	0.2099
Rwork	0.207
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.400
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.800	2.430
High resolution limit [Å]	2.300	2.300
Rmerge	0.140
Number of reflections	20402	130
<I/σ(I)>	4.3	1.7
Completeness [%]	90.9
Redundancy	2
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	12% PEG4000, 0.1M Tris pH8.5, 0.1M NaI