10XU

Structure of amplified aSyn filament by using seed amplification assay (SAA) from MSA patient CSF.

Replaces:  8UKA

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	HELICAL
Resolution (Å)	3.18
Software	RELION,PHENIX
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION