10XD
Rhodococcus ruber DnaA DI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.904, 63.904, 63.272 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.950 - 1.950 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.407 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.950 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.070 | 1.576 |
| Rpim | 0.016 | 0.361 |
| Number of reflections | 11280 | 807 |
| <I/σ(I)> | 23.6 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.5 | 18.9 |
| CC(1/2) | 0.999 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.3 M Ammonium citrate tribasic dihydrate, pH 7.0; 0.1M Bis-tris propane, pH 7.0 |
