10XC

Nocardia asteroides DnaA DI in the P4(1)22 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU PhotonJet-S
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-12-17
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 41 2 2
Unit cell lengths	41.538, 41.538, 94.783
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.510 - 1.200
R-factor	0.15
Rwork	0.148
R-free	0.18010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.238
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.510	1.220
High resolution limit [Å]	1.200	1.200
Rmeas	0.140	1.428
Rpim	0.027	0.357
Number of reflections	26871	1309
<I/σ(I)>	14.6	2.2
Completeness [%]	100.0
Redundancy	23.9
CC(1/2)	0.999	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM Sodium citrate/Citric Acid, pH 5.0; 800 mM Ammonium Sulfate