10XB

Nocardia asteroides DnaA DI protein in the I23 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-12
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	I 2 3
Unit cell lengths	100.718, 100.718, 100.718
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.610 - 2.400
R-factor	0.2148
Rwork	0.213
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.561
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.610	2.490
High resolution limit [Å]	2.400	2.400
Rmeas	0.080	1.428
Rpim	0.018	0.327
Number of reflections	6797	697
<I/σ(I)>	23	2
Completeness [%]	100.0	100
Redundancy	19.2	19
CC(1/2)	1.000	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.7 M Ammonium citrate tribasic dihydrate; 0.1 M Tris, pH 8.5