10XA

Nocardia asteroides DnaA DI in the P31(1)21 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU PhotonJet-S
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-25
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 31 2 1
Unit cell lengths	46.751, 46.751, 88.743
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.380 - 1.250
R-factor	0.1491
Rwork	0.148
R-free	0.16810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.385
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.380	1.270
High resolution limit [Å]	1.250	1.250
Rmeas	0.047	0.596
Rpim	0.008	0.226
Number of reflections	31853	1524
<I/σ(I)>	31.5
Completeness [%]	99.9
Redundancy	21.9	6.3
CC(1/2)	1.000	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1260 mM ammonium sulfate; 100 mM Tris base/hydrochloric acid, pH 8.5; 200 mM Lithium Sulfate