10WY

Rhodococcus hoagii DnaA DI in the P4(3)2(1)2 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-06
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.00003
Spacegroup name	P 43 21 2
Unit cell lengths	60.646, 60.646, 132.457
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.730 - 1.600
R-factor	0.1757
Rwork	0.174
R-free	0.19780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.731
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.730	1.630
High resolution limit [Å]	1.600	1.600
Rmeas	0.055	0.496
Rpim	0.016	0.188
Number of reflections	33536	1608
<I/σ(I)>	20	1.7
Completeness [%]	100.0	99.5
Redundancy	11.6	6.5
CC(1/2)	0.999	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2.2 M Ammonium Sulfate, 0.2 M Lithium Sulfate, 0.1 M Tris, pH 8.33.