10UY

Cohesin domain number 4 from gene locus Rcal_2942 of Ruminococcus callidus, a type 4 cohesin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979200
Spacegroup name	P 21 21 21
Unit cell lengths	33.700, 36.990, 109.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.670 - 1.100
R-factor	0.1355
Rwork	0.133
R-free	0.16280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.957
Data reduction software	XDS (20250714)
Data scaling software	XSCALE (20250714)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.845	1.130
High resolution limit [Å]	1.100	1.100
Rmeas	0.079	0.833
Number of reflections	547047	13389
<I/σ(I)>	15.51	2.32
Completeness [%]	91.6	52.2
Redundancy	10.5	6.2
CC(1/2)	0.998	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1M Sodium acetate pH 4.6, 8% (w/v) PEG 4000