10UX

Cohesin domain number 2 from gene locus Rcal_2942 of Ruminococcus callidus, a type 4 cohesin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979200
Spacegroup name	P 1 21 1
Unit cell lengths	40.010, 147.530, 61.240
Unit cell angles	90.00, 96.07, 90.00

Refinement procedure

Resolution	46.960 - 1.900
R-factor	0.1729
Rwork	0.171
R-free	0.19510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.811
Data reduction software	XDS (20241002)
Data scaling software	XSCALE (20241002)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.290	56.290	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.108	0.061	0.785
Rmeas	0.119	0.067	0.911
Number of reflections	53864	634	3896
<I/σ(I)>	11.22
Completeness [%]	97.2
Redundancy	5.8
CC(1/2)	0.997	0.995	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Potassium formate, 20 % w/v PEG 3350