10UW

Cohesin domain number 1 from gene locus Rcal_2942 of Ruminococcus callidus, a type 4 cohesin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979200
Spacegroup name	P 21 21 21
Unit cell lengths	121.920, 121.470, 60.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.480 - 1.900
R-factor	0.1803
Rwork	0.178
R-free	0.20010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.844
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	54.480	54.480	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.108	0.066	0.873
Rmeas	0.120	0.074	0.973
Number of reflections	70378	886	5039
<I/σ(I)>	9.72
Completeness [%]	98.8
Redundancy	5
CC(1/2)	0.994	0.993	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1.6 M Magnesium sulfate, 0.1 M MES 6.5