10UV

Cohesin domain number 2 from gene locus Rcal_2938 of Ruminococcus callidus, a type 4 cohesin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979200
Spacegroup name	C 2 2 21
Unit cell lengths	86.830, 88.870, 82.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.110 - 2.000
R-factor	0.1823
Rwork	0.179
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.833
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.110	62.110	2.050
High resolution limit [Å]	2.000	8.950	2.000
Rmerge	0.082	0.039	0.929
Rmeas	0.089	0.043	1.012
Number of reflections	21748	283	1563
<I/σ(I)>	14.32
Completeness [%]	99.5
Redundancy	6.5
CC(1/2)	0.998	0.998	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M HEPES 7.0, 10 % w/v PEG 600