10UT

Cohesin domain number 1 from gene locus Rcal_2938 of Ruminococcus callidus, a type 4 cohesin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979200
Spacegroup name	P 41
Unit cell lengths	52.340, 52.340, 101.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.340 - 1.600
R-factor	0.179
Rwork	0.177
R-free	0.20010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.811
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.340	52.340	1.640
High resolution limit [Å]	1.600	7.150	1.600
Rmerge	0.140	0.093	1.528
Rmeas	0.143	0.095	1.568
Number of reflections	36129	422	2684
<I/σ(I)>	13.9
Completeness [%]	100.0
Redundancy	20.8
CC(1/2)	0.999	0.999	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.14 M Calcium chloride dihydrate, 0.07 M Sodium acetate 4.6, 14 % v/v 2-Propanol, 30 % v/v Glycerol