10US

Cohesin domain number 1 from gene locus Rcal_0670 of Ruminococcus callidus, a type 5 cohesin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979200
Spacegroup name	P 21 21 21
Unit cell lengths	42.560, 43.950, 267.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.540 - 2.100
R-factor	0.196
Rwork	0.193
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.862
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.540	44.540	2.160
High resolution limit [Å]	2.100	9.400	2.100
Rmerge	0.210	0.047	1.330
Rmeas	0.221	0.050	1.407
Number of reflections	30304	419	2151
<I/σ(I)>	9.78
Completeness [%]	99.9
Redundancy	10.3
CC(1/2)	0.995	0.998	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M Sodium citrate pH 5.5, 20 % w/v PEG 3000