10UR

Cohesin domain number 4 from gene locus Rcal_0153 of Ruminococcus callidus, a type 5 cohesin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979200
Spacegroup name	C 2 2 21
Unit cell lengths	42.530, 84.480, 118.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.780 - 1.800
R-factor	0.1938
Rwork	0.189
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.446
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.780	39.780	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.128	0.052	0.591
Rmeas	0.138	0.055	0.648
Number of reflections	19021	259	976
<I/σ(I)>	11.63
Completeness [%]	94.1
Redundancy	8.3
CC(1/2)	0.991	0.998	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	293	0.2 M Magnesium formate pH 5.9, 20% PEG 3350