10UQ

Cohesin domain number 3 from gene locus Rcal_0153 of Ruminococcus callidus, a type 5 cohesin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979200
Spacegroup name	P 1 21 1
Unit cell lengths	92.110, 172.020, 92.190
Unit cell angles	90.00, 106.19, 90.00

Refinement procedure

Resolution	88.460 - 2.800
R-factor	0.1868
Rwork	0.183
R-free	0.20140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.834
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	88.460	88.460	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.201	0.062	1.747
Rmeas	0.221	0.069	1.916
Number of reflections	67393	773	4981
<I/σ(I)>	8.5
Completeness [%]	99.4
Redundancy	5.8
CC(1/2)	0.988	0.994	0.277

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M di-Potassium phosphate 2.2 M Ammonium sulfate