10PW

Crystal structure of Glutathione Transferase from Shrimp Litopenaeus vannamei in complex with silver ions and a molecules of Glutathione binding in G-site and H-site

Replaces:  7SO8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-26
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	57.390, 93.020, 169.110
Unit cell angles	90.00, 90.75, 90.00

Refinement procedure

Resolution	56.370 - 2.200
R-factor	0.195
Rwork	0.192
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.139
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.370	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.160	0.610
Rmeas	0.200	0.750
Rpim	0.110	0.420
Number of reflections	88718	8800
<I/σ(I)>	6.6	1.98
Completeness [%]	98.2	97.52
Redundancy	3.1	3.1
CC(1/2)	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	277.15	100 MM AMMONIUM SULPHATE, 100 BIS-TRIS, PH 6.5, 30% PEG 3350, 300 UM SILVER NITRATE