10OO

FGFR2 mutant D650V with compound 4 (AZD3463)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-08-08
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	66.932, 77.985, 116.071
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.020 - 1.850
R-factor	0.21317
Rwork	0.211
R-free	0.25576
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.287
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.023	1.950
High resolution limit [Å]	1.847	1.850
Rmerge	0.163	0.815
Number of reflections	52406	7618
<I/σ(I)>	6.42	1.36
Completeness [%]	99.0	96.9
Redundancy	12.54	11.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	30% PEG 4,000, 200 mM Lithium Sulfate, 100 mM TRIS pH 8.5