10OJ

The crystal structure of apo phosphofructokinase from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978560
Spacegroup name	P 21 2 21
Unit cell lengths	76.699, 90.847, 103.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.420 - 2.600
R-factor	0.2313
Rwork	0.230
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.420	2.470
High resolution limit [Å]	2.380	2.380
Rpim	0.148
Number of reflections	22948	3088
<I/σ(I)>	7
Completeness [%]	100.0	100
Redundancy	13.3
CC(1/2)	0.995	0.123

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris, pH 8.5, 0.3 M magnesium formate dihydrate