10LG

Crystal structure of Streptococcus thermophilus SHP pheromone receptor Rgg3 in complex with Rgg3bp13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU PhotonJet-S
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-11-17
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54056
Spacegroup name	P 32 2 1
Unit cell lengths	111.678, 111.678, 50.749
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.940 - 1.870
R-factor	0.1865
Rwork	0.183
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.676
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHENIX
Refinement software	PHENIX (2.0_5904)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.940	19.940	1.940
High resolution limit [Å]	1.870	4.090	1.870
Rpim	0.045	0.013	0.295
Number of reflections	30196	2996	2922
<I/σ(I)>	16.08	55.83	1.11
Completeness [%]	99.4	98.6	95.9
Redundancy	10.4	9.5	7.3
CC(1/2)	0.998	0.999	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	100 mM sodium citrate pH 5.2, 300 mM Na/K tartrate, and 1.4 M ammonium sulfate