10LE
X-ray structure of the Bacteroides fragilis Nramp/MntH divalent transition metal transporter WT in an inward-open, manganese-bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 68.853, 101.910, 118.895 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.840 - 2.400 |
| R-factor | 0.2402 |
| Rwork | 0.238 |
| R-free | 0.27490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.423 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (2.0_5936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.840 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.381 | |
| Rmeas | 0.455 | |
| Rpim | 0.248 | |
| Number of reflections | 33378 | 33378 |
| <I/σ(I)> | 4.1 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.2 | 6.2 |
| CC(1/2) | 0.980 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 298 | 0.2 M ammonium sulfate, 0.02 M sodium chloride, 0.02 M sodium acetate pH 4.0, 33% PEG 200 |
